All products Antibodies Hsp70 [SA0379]

Hsp70 [SA0379]

Catalog Number：OM626536

  • Fig1: Western blot analysis of Hsp70 on different cell lysates using anti-Hsp70 antibody at 1/1,000 dilution. Positive control: Lane 1: MCF-7 Lane 2: HCT116

  • Fig2: ICC staining Hsp70 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Fig3: ICC staining Hsp70 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Fig4: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Hsp70 antibody. Counter stained with hematoxylin.

  • Fig5: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Hsp70 antibody. Counter stained with hematoxylin.

  • Fig6: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Hsp70 antibody. Counter stained with hematoxylin.

  • Fig7: Immunohistochemical analysis of paraffin-embedded mouse prostate tissue using anti-Hsp70 antibody. Counter stained with hematoxylin.

  • Fig8: Flow cytometric analysis of Hela cells with Hsp70 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Product Profile

Product Name Hsp70 [SA0379]
Antibody Type Primary Antibodies
Product description 
The 70 kilodalton heat shock proteins (Hsp70s) are a family of conserved ubiquitously expressedheat shock proteins. Proteins with similar structure exist in virtually all living organisms. The Hsp70s are an important part of the cell's machinery for protein folding, and help to protect cells from stress.  When not interacting with a substrate peptide, Hsp70 is usually in an ATP bound state. Hsp70 by itself is characterized by a very weak ATPase activity, such that spontaneous hydrolysis will not occur for many minutes. As newly synthesized proteins emerge from the ribosomes, the substrate binding domain of Hsp70 recognizes sequences of hydrophobic amino acid residues, and interacts with them.  This spontaneous interaction is reversible, and in the ATP bound state Hsp70 may relatively freely bind and release peptides. However, the presence of a peptide in the binding domain stimulates the ATPase activity of Hsp70, increasing its normally slow rate of ATP hydrolysis.
Immunogen 
recombinant protein

Key Feature

Clonality Monoclonal
Isotype IgG
Host Species Recombinant rabbit
Tested Applications

WBICC/IFIHCFC
WB:1:1,000-1:5,000
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:10-1:100
Species Reactivity

HumanMouseRat
Concentration 1 mg/mL.

Target Information

Alternative Names
DnaK type molecular chaperone HSP70 1 antibody Epididymis secretory protein Li 103 antibody FLJ54303 antibody FLJ54370 antibody FLJ54392 antibody FLJ54408 antibody FLJ75127 antibody Heat shock 70 kDa protein 1 antibody Heat shock 70 kDa protein 1/2 antibody Heat shock 70 kDa protein 1A/1B antibody Heat shock 70kDa protein 1A antibody Heat shock 70kDa protein 1B antibody Heat shock induced protein antibody HEL S 103 antibody HSP70 1 antibody HSP70 1B antibody HSP70 2 antibody HSP70-1/HSP70-2 antibody HSP70-1A antibody HSP70.1 antibody HSP70.1/HSP70.2 antibody HSP70I antibody HSP71_HUMAN antibody HSP72 antibody HSPA1 antibody HSPA1A antibody HSPA1B antibody
Molecular Weight(MW) 70 kDa
Cellular Localization Cytoplasm

Database Links

SwissProt ID P0DMV9
P0DMV8
P17879
Q61696
Q07439

Application

  • Application

    Fig1: Western blot analysis of Hsp70 on different cell lysates using anti-Hsp70 antibody at 1/1,000 dilution. Positive control: Lane 1: MCF-7 Lane 2: HCT116

  • Application

    Fig2: ICC staining Hsp70 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Application

    Fig3: ICC staining Hsp70 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Application

    Fig4: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Hsp70 antibody. Counter stained with hematoxylin.

  • Application

    Fig5: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Hsp70 antibody. Counter stained with hematoxylin.

  • Application

    Fig6: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Hsp70 antibody. Counter stained with hematoxylin.

  • Application

    Fig7: Immunohistochemical analysis of paraffin-embedded mouse prostate tissue using anti-Hsp70 antibody. Counter stained with hematoxylin.

  • Application

    Fig8: Flow cytometric analysis of Hela cells with Hsp70 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Positive Control HCT116, MCF-7, Hela, human breast carcinoma tissue, mouse prostate tissue, mouse testis tissue, mouse brain tissue.
Application Notes
WB:1:1,000-1:5,000
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:10-1:100

Additional Information

Form Liquid
Storage Instructions Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage Buffer 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

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  • Catalog Number: OM626536
  • Price: $386.00
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  • Lead time: 4~6 Week

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